조성미 Seong Mi Jo , 김정은 Jung Eun Kim , 이남호 Nam Ho Lee

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Abstract

In this study, the anti-inflammatory and anti-bacterial activities of the extracts from the leaves of the Hydrangea petiolaris were identified, and the chemical structure was identified by separating the active ingredient. As the result of the anti-inflammatory activity experiment using RAW 264.7 cells, it was confirmed that the n-hexane (Hex) and ethyl acetate (EtOAc) fractions inhibited the production of nitric oxide (NO) and the expression of iNOS protein in a concentration-dependent manner without cytotoxicity. In addition, the n-Hex and EtOAc fractions reduced the production of pro-inflammatory cytokines (TNF-α, IL-1β and IL-6). Upon the anti-bacterial tests using Staphylococcus epidermidis and Cutibacterium acnes, the extract, n-Hex, EtOAc and n-butanol (BuOH) fractions showed potent activities. In order to isolate the active constituents, the n-Hex and EtOAc fractions were further purified to afford four phytochemicals; phytol (1), corosolic acid (2), asiatic acid (3) and 1-O-p-coumaroyl-β-D-glucopyranoside (4). All of the compounds 1 - 4 were isolated for the first time from this plant. In addition, the contents of isolated compounds were determined by HPLC and the quantity of phytol (1) was 27.8 mg/g for the 70% EtOH extract. Based on the above research results, it is believed that it will be possible to develop a natural cosmetic material that has anti-inflammatory and anti-bacterial effects using the extract of H. petiolaris leaves.

Key Words

Hydrangea petiolaris, anti-inflammation, anti-bacterial activity, isolation

홍경식 Gyeong Sik Hong , 최정연 Jeong Yeon Choi , 최진현 Jin Hyun Choi

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Abstract

Recently, since atopic dermatitis is sensitive to skin irritation, it has been suggested that the development of a patch that can effectively exhibit adhesion and absorption to a specific local area while minimizing skin irritation, and capable of appropriate drug release should be given priority. In this study, we tried to develop a hydrogel patch that minimizes skin irritation, adheres effectively to a specific area, and promotes absorption. The atopic patch was formulated into a super-absorbent hydrogel sheet using a freeze drying method. Cell viability assay was carried out using keratinocytes (HaCaT cell) and fibroblasts (L929 cells). In order to investigate the physical properties, FT-IR, FE-SEM, porosity analysis and swelling behavior were investigated. As a result, the newly prepared HA-Dex hydrogel patch was verified by biocompatibility and physical evaluation. In addition, the manufactured hydrogel patch has sufficient moisture absorption capacity and can relieve itching of atopic skin, and is expected to be applied to various drug delivery products for the treatment of atopic dermatitis in the future.

Key Words

atopic dermatitis, hydrogel patch, hyaluronic acid, dextran, BDDE

성미경 Mi Kyung Sung , 강정욱 Jung Wook Kang , 정윤주 Yoon Ju Jeong , 김봉준 Bong Jun Kim , 조항의 Hang Eui Cho , 조현대 Hyun Dae Cho

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Abstract

In this study in order to develop new approaches we investigated using high voltage pulsed electric fields (PEF) technology to reduce the risks, protect the phyto-constituents and improve skin biological activities. After preparing a Chaga mushroom (Inonotus obliquus) extracts pretreated with PEF, components measurement and skin efficacy evaluation were performed. As a result of the content measurement, the content of polysaccharide and polyphenol were higher in the order of extracts treated with 50 Hz and 25 Hz at 0.5 kV/cm, and the content of protein was the highest in extracts treated with 25 Hz at 0.5k V/cm. Similar to the results of the polyphenol measurements, extracts treated with 25 Hz and 50 Hz at 0.5 kV/cm showed leading DPPH scavenging ability. The cell protection effect against sodium dodecyl sulfate (SDS) and UVB was finest in extracts treated with 25 Hz at 0.5 kV/cm, which had the highest protein content. And the hyaluronic acid synthesis was leading in extracts treated with 50 Hz and 100 Hz at 0.5 kV/cm. Therefore, the active ingredient of the high-voltage PEF pre-treatment Chaga mushroom extract can be developed as a functional material with cell protection and moisturizing effect, and such green technology is expected to be used in various fields of cosmetics and material development.

Key Words

high voltage pulsed electric fields, Chaga mushroom, extraction, skin effects, cosmetics

맹지혜 Jihye Maeng , 남개원 Gaewon Nam

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Abstract

Using Fervidobacterium islandicum AW-1, polar low molecular weight keratin peptides were produced and confirmed through factors related to the skin elasticity. As a result of confirming the cytotoxicity and collagen synthesis ability according to the concentration of the polar low molecular weight keratin peptide in human fibroblasts, it was confirmed that the cytotoxicity did not appear and the collagen synthesis in human fibroblasts was increased. A mask pack containing a polar low-molecular weight keratin peptide was used, and a test product was used for 4 weeks in 22 healthy women subjects. As a result, it showed statistically significant effects on skin elasticity, skin torsion elasticity, skin color and moisture improvement. Through this test, it was confirmed that the polar low-molecular keratin peptide can be used as a cosmetic ingredient that helps improve skin elasticity.

Key Words

Fervidobacterium islandicum AW-1, polar low molecular keratin peptide, skin elasticity, mask pack, bioactive peptide

전선홍 Seon Hong Jeon , 오솔라 Sol La Oh , 김소정 So Jeong Kim , 전보희 Bo Hee Jeon , 성진영 Jin Young Sung , 김용민 Yong Min Kim

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Abstract

Reactive oxygen species (ROS) plays an important role in maintaining homeostasis. However, excessive ROS production damages cellular components such as proteins, lipids, and nucleic acids and promotes skin aging. In this study, we confirmed the antioxidant effect of CSYJE to prevent excessive oxidative stress. First, DPPH and ABTS assays were performed to confirm the antioxidant effect of CSYJE and the radical scavenging activity was confirmed depending on the concentration. As a result of performing the MTT assay to confirm the cell viability, it was confirmed that there was no cytotoxicity at a concentration of 1,000 μg/mL. As a result of western blotting to confirm the expression levels of the antioxidant-related proteins nuclear-E2-related factor 2 (Nrf2) and Heme oxygenase-1 (HO-1), it was confirmed that the expression was increased in a concentration-dependent manner. After inducing ROS with lipopolysaccharide (LPS), an intracellular ROS-causing substance, DCF-DA was performed to confirm the inhibitory effect of ROS production, and the inhibition of ROS production was confirmed to concentration-dependent. Real-time RT-PCR was performed to confirm the mRNA expression level of inflammatory cytokines and inflammatory mediator caused by ROS generation, mRNA expression was reduced in a dose dependent manner. Therefore, this study confirmed the antioxidant effect of CSYJE through the Nrf2/HO-1 signaling pathway, which suggests that CSYJE can be used as an antioxidant cosmetic material by inhibiting free radicals.

Key Words

anti-oxidative, Chungsimyeonja-um, CSYJE, HO-1, Nrf2, RAW 264, 7

서영경 Young Kyoung Seo , 백지훈 Ji Hwoon Baek , 부용출 Yong Chool Boo , 고재숙 Jae Sook Koh

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Abstract

The body odor as well as the skin changes has become an important issue. Humans leave respective specific odors dependent on sex and age; The odor has been known to affect social relationship of human. In this study, we wanted to confirm the possibility as parameters to evaluate body odor into existing odor evaluation methods by experts. The 15 subjects, aged from 50 to 61 years wore cotton t-shirts for 72 hours and collected body odor. The cotton t-shirts containing body odor were used for sensory evaluation and EEG measurement by the odor experts. In order to evaluate body odors of each subject, an odor sensory evaluation and electroencephalogram (EEG) were conducted by odor exports and the correlation in between two assessments was analyzed. Pearson correlation analysis shows negative correlation of the sensory evaluation versus ‘Excitement’ in EEG parameter (r = - 0.649, p = 0.009) and positive correlation of the sensory evaluation versus ‘Stress’ in EEG parameter (r = 0.704, p = 0.003). In conclusion, it is considered that the evaluation of body odor through EEG measurement can be used as a method to complement the odor evaluation by experts.

Key Words

body odor, sensory evaluation, electroencephalogram, EEG

이경주 Kyung Ju Lee , 장욱주 Wookju Jang , 김유아 You Ah Kim , 박병준 Byoung Jun Park , 강학희 Hakhee Kang

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Abstract

Aloe vera (Aloe barbadensis Miller) has been used since ancient times to improve various skin diseases such as burns, wounds, and eczema. It has been reported that Aloe vera contains vitamin, enzyme, mineral, sugar, phenolic compound, fatty acid and amino acid. Aloe vera changes its color from green to red under the extreme thermal and arid climate to protect itself. These morphological changes induce variation of composition such as increasing of aloe-emodin content. Aloe-emodin is one of the major anthraquinone in aloe family plants. Since aloe-emodin contains a polyphenolic structure, this compound may be responsible for the reported antioxidant and anti-inflammatory effects of aloe. However, there is no research on the process of increasing the compounds of Aloe vera. Therefore, the purpose of this study is to develop a pink aloe manufacturing process that increases the aloe-emodin content and enhances the antioxidant and anti-inflammatory activities of aloe. As a result of heating aloe under appropriate conditions, pink aloe increased aloe-emodin content compared to general aloe, and exhibited effects such as increasing antioxidant activity, inhibiting NO production, and promoting cell regeneration. Through this study, the applicability of pink aloe as a new anti-aging material in the cosmetic field was confirmed.

Key Words

Aloe vera, Pink aloe, antioxidant, anti-inflammatory, wound healing

고은아 Eun Ah Ko , 남승희 Seung-hee Nam , 정하나 Hana Jeong , 김보윤 Bo Yun Kim , 곽상화 Sang Hwa Kwak , 김선영 Sunyoung Kim , 홍인기 In Ki Hong , 강학희 Hakhee Kang

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Abstract

In this study, in order to increase the utilization of Citrus junos seeds, which account for 13% of the weight ratio of Citrus junos ripened fruit, but are mostly discarded and not utilized, the efficacy of skin beauty of Citrus junos seed oil extracted by cold pressing was studied. Citrus junos seed oil was found to contain approximately 74% of unsaturated fatty acids consisting mainly of oleic acid and linoleic acid, and limonene, which is mainly contained in Citrus junos peel, contained a very low content of about 0.0187%. As a result of evaluating the DPPH radical scavenging activity of Citrus junos seed oil, 26% of DPPH radical scavenging ability was confirmed at 5% concentration of Citrus junos seed oil. To confirm the anti-inflammatory effect, as a result of testing RAW 264.7 cytotoxicity test and NO production for Citrus junos seed oil, NO production was suppressed by 53% at a concentration of 0.05% that does not show cytotoxicity. In addition, in the RBL-2H3 cytotoxicity and β-hexosaminidase release inhibitory efficacy test for anti-allergic efficacy confirmation, it was confirmed that β-hexosaminidas release was suppressed by 26% at a concentration of 0.05% that did not show cytotoxicity. Lastly, in the human skin application test result of O/W emulsion containing 5% of Citrus junos seed oil, it showed higher skin moisturizing effect than the control emulsion containing the same amount of caprylic/capric triglyceride. Therefore, it is thought that Citrus junos seed oil might be used as a excellent skin care material.

Key Words

Citrus junos, unsaturated fatty acid, anti-inflammation, anti-allergy, moisturization

임병택 Byung Tack Lim , 서홍안 Hong An Seo , 송상훈 Sang-hun Song , 손성길 Seong Kil Son , 강내규 Nae-gyu Kang

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Abstract

Treatment for beauty using oxidizing agents damages hair with inducing structural alteration in cuticle layer, degradation of protein, and loss of lipid. This study connects a frictional coefficient upon the damaged hair by an instrumental test to the texture test by human being, and considered a moisture as a factor of the damage. A friction coefficient has been measured upon the hair with successive treatment of dye, perm, and bleach. The friction coefficient from the hair dye-treated three times was defined with 0.60, where 58% of answerer indicated an initial damage point as the hairs of iteration of dye-treatment increased. Even bleach treated three times results in 0.84 of friction coefficient corresponding to 88% of answerer attributed the hair to an initially damaged hair. In order to figure out a lipid loss in hair for human being to respond damage, a friction coefficient of the hair was controlled by removing 18-methyleicosanoic acid (18-MEA). The initial damage has been recognized by 0.60 of the friction coefficient for the 68% of answerer. Since moisture is the largest portion of the components in hair, moisture analysis has been performed to study a relationship between texture of damage and the friction coefficient from an instrumental evaluation. As an iteration of dye increases, the hair became hydrophilic with smaller contact angle. It is found that a damaged hair by dyeing possessed more than 0.42% of moisture compared to a healthy hair. Finally, it is elucidated that an increase of moisture in hair induced higher adhesive force corresponding to the friction coefficient, and the friction coefficient above 0.6 is attributed to the preception of hair damage.

Key Words

friction, hair, damage, emotional evaluation

김혜진 Hye Jin Kim , 김선영 Sunyoung Kim , 이설훈 Seol-hoon Lee

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Abstract

The barrier structure of the skin’s epidermis is a key structure to prevent the loss of water inside the body and the invasion of foreign substances, and is composed of keratinocytes and intercellular lipids. At this time, the intercellular lipids of the skin barrier has the strongest structure when packed in an orthorhombic structure. However, it is damaged by various external causes and changes to a hexagonal structure. This change in physical structure can be analyzed non-invasively by analyzing the signal of the CH₂-CH₂ scissoring band of lipids using FT-IR. In this study, SDS was treated on porcine skin to construct a skin barrier damage model, and the degree of change in packing structure was quantified by analyzing FT-IR signals. We then judged whether the barrier of the damage model was recovered according to the treatment of the cosmetic formulation. From these results, an indirect method of measuring the water evaporation of the skin barrier to date can be supplemented. In addition, physical changes in the structure of the skin barrier can be utilized in a direct and efficient manner to identify the function and verify the formulation of various materials.

Key Words

skin barrier, lipid packing, orthorhombic, non-invasive analysis, ceramide