The Effects of Kinetin on Moisturization, Skin Barrier, and Anti-inflammation 키네틴의 보습, 피부장벽 및 항염증에 미치는 효과
강준철 Jun-chul Kang , 김지혜 Jihye Kim , 김선화 Seonhwa Kim , 손동주 Dong Ju Son , 박현철 Hyun-chul Park
50(4) 301-307, 2024
강준철 Jun-chul Kang , 김지혜 Jihye Kim , 김선화 Seonhwa Kim , 손동주 Dong Ju Son , 박현철 Hyun-chul Park
DOI:
Abstract
Kinetin, known as N6-furfuryladenine, was discovered as a plant cytokinin in 1955, and various effects such as anti-aging, light protection, and skin barrier functions have been reported. In this study, we evaluated kinetin for its moisturizing, skin barrier function and anti-inflammatory effects on HaCaT cells and RAW 264.7 cells. At the first, we assessed the cytotoxicity of kinetin in HaCaT and RAW 264.7 cells using WST-8 kit. Based on this result, the other experiment was conducted at concentrations where the cell viability was 90% or higher. Kinetin significantly induced the gene expression of filaggrin (FGL), aquaporin3 (AQP3), hyaluronic acid synthase HAS2, and HAS3 in HaCaT cells. It was also confirmed that kinetin significantly inhibited the production of nitric oxide (NO) in RAW 264.7 cells. These results suggest that kinetin can be used as a functional material for cosmetics for skin moisturizing, skin barrier, and anti-inflammatory.
Key Words
kinetin, skin, moisturizing, skin barrier, anti-inflammatory
|
A Study on Antioxidant, Anti-inflammatory, Sebum Control, Moisturizing and Dermal Papilla Cells Activation Efficacy of Samdachal Extract 삼다차조 추출물의 항산화, 항염, 피지 조절, 보습 및 모유두세포 활성화 효능에 대한 연구
조성미 Seong Mi Cho , 김혜빈 Hye Bin Kim , 김건우 Keon Woo Kim , 문지영 Ji Young Moon , 김정미 Jeong Mi Kim , 박진오 Jin Oh Park , 이혜자 Hye Ja Lee
50(4) 309-317, 2024
조성미 Seong Mi Cho , 김혜빈 Hye Bin Kim , 김건우 Keon Woo Kim , 문지영 Ji Young Moon , 김정미 Jeong Mi Kim , 박진오 Jin Oh Park , 이혜자 Hye Ja Lee
DOI:
Abstract
This study was conducted to propose Samdachal as a material related to hair loss and scalp environment improvement. Antioxidant, anti-inflammatory, sebum control, human dermal papilla cell activation, and moisturizing efficacy were confirmed using Samdachal extract (SE), and the SC50 value of SE was 0.33 mg/mL as a result of the ABTS+ radial scavenging activity experiment. In the anti-inflammatory tests using Griess reagent in lipopolysaccaride (LPS)-induced RAW 264.7 cells, it was confirmed that SE significantly inhibited NO produced excessively due to LPS. As a result of the sebum control test using kit in palmitic acid-induced human sebocytes cells, it was confirmed that SE significantly inhibited the production of increased lipid due to palmitic acid. In addition, for the cell proliferation test using EZ-Cytox reagent in human dermal papilla cells (HFDPC), SE was confirmed to have the effect of cell proliferation effect in HFDPC. As a result of measuring the production amount of the vascular endothelial growth factor (VEGF), a hair growth factor, using the ELISA Kit in HFDPC, SE significantly increased the production amount of VEGF. Finally, as a result of measuring the amount of hyaluronic acid (HA) production using the ELISA kit in the HaCaT cell, the amount of HA production was significantly increased. Based on these results, it is considered that SE could be used as a natural cosmetic material that has the effect of improving hair loss and scalp environment.
Key Words
Samdachal, hair loss, scalp, anti-inflammatory, lipid
|
The Protective Effects of Puffing-processed Coptis japonica Root Extract on the Hs68 Cells Against Blue Light Emitted from Digital Devices 블루라이트에 대한 팽화처리된 황련뿌리 추출물의 Hs68 세포 보호효과
강준철 Jun-chul Kang , 윤유경 Yoo-gyeong Yoon , 김소희 So Hui Kim , 손동주 Dong Ju Son , 홍진태 Jin Tae Hong , 박현철 Hyun-chul Park
50(4) 319-325, 2024
강준철 Jun-chul Kang , 윤유경 Yoo-gyeong Yoon , 김소희 So Hui Kim , 손동주 Dong Ju Son , 홍진태 Jin Tae Hong , 박현철 Hyun-chul Park
DOI:
Abstract
Blue light (380 ~ 500 nm) has the highest energy among visible wavelengths and is known to induce free radicals, inflammation, and apoptosis in skin cells. In our search for natural materials with protective effects against blue light, we found that Coptis japonica root extract demonstrates a significant protective effect. C. japonica is a perennial medicinal plant grown in Asia, known for its anti-inflammatory, antioxidant, and antibacterial properties. In this study, we evaluated its efficacy against the harmful effects of blue light (BL) using gun-puffed C. japonica (PCE). The antioxidant effect of PCE was confirmed by DPPH, and treatment of Hs68 cells before and after blue light exposure confirmed that PCE provided cytoprotection, reduced intracellular free radicals, and inhibited DNA damage and apoptosis. These findings indicate that PCE may serve as a promising candidate for a photoprotective agent to prevent skin cell damage from blue light exposure.
Key Words
Coptis japonica, blue light irradiation, human dermal fibroblast, apoptosis, photoprotection
|
Identification of Whitening Effects of Alpinia officinarum Extracts 고량강 추출물의 미백 기능 규명
김영주 Young Joo Kim
50(4) 327-334, 2024
김영주 Young Joo Kim
DOI:
Abstract
In this study, we investigated the inhibitory effect of Alpinia officinarum (A. officinarum), a Korean herbal medicine ingredient, on melanogenesis. For this research, A. officinarum was extracted using 70% ethanol, and its whitening functionality was evaluated by treating B16F10 cells, a mouse-derived melanogenic cell line. To confirm the whitening effect, we examined melanin production inhibition, the activity and expression inhibition of tyrosinase, and changes in the transcriptional activity of microphthalmia-associated transcription factor (MITF). The results showed that the extract of A. officinarum (100 μg/mL) inhibited melanin production by 57.63%, which was associated with a concentration-dependent decrease in both the activity and expression of tyrosinase. Additionally, the expression of MITF, a transcription factor for tyrosinase, also decreased, indicating that the whitening efficacy of the A. officinarum extract occurs through the regulation of transcription factor activity. These findings suggest that A. officinarum extract possesses significant whitening functionality and may be utilized as a novel whitening functional material in the future. This study provides fundamental data on the whitening effects of A. officinarum and contributes to exploring modern applications of traditional herbal materials.
Key Words
Alpinia officinarum, tyrosinase, melanin, whitening
|
Senolytic and Senomorphic Effects of Lactobacillus plantarum DS0037 Derived Exosome-like Nanovesicles Lactobacillus plantarum DS0037 유래 엑소좀 유사 나노베지클의 세놀리틱 및 세노모르픽 효과
태수길 Sookil Tae , 이혜연 Hye Yeon Lee , 오승익 Seung-ick Oh
50(4) 335-348, 2024
태수길 Sookil Tae , 이혜연 Hye Yeon Lee , 오승익 Seung-ick Oh
DOI:
Abstract
In this study, to develop natural microbial-derived anti-aging materials, we isolated more than 100 anaerobic microorganisms from fresh ginseng and measured their senescent cell-specific anti-aging activities, and identified a novel Lactobacillus plantarum DS0037 (L. plantarum DS0037) with the best senolytic activity. After manufacturing exosome-like nanovesicles from 6 selected L. plantarum and 4 existing deposit strains, the cell viability was confirmed using a stress-induced early aging cell model, and it was confirmed that the survival rate in aging cells was suppressed by 54.5% compared to young cells in the L. plantarum DS0037 exosome-like nanovesicle treatment zone. This senescent cell-specific inhibitory effect was confirmed to be regulated through selectively killing senescent cells such as ABT-737. Moreover, the ELNs of L. plantarum DS0037 downregulated the expression of MMP-1 and IL-6, both highly expressed in senescent cells, while upregulated the expression of Col1A1 as well as enhanced the expression of procollagen proteins in senescent cells. When analyzing variables related to improving skin elasticity in clinical evaluation of L. plantarum DS0037 derived exosome-like nanovesicles raw materials, it was confirmed that the improvement was made after both 1 and 2 weeks of use. These results suggest that L. plantarum DS0037 exosome-like nanovesicles can improve the function of aging skin cells and can be used as effective cosmetic materials with anti-aging activity in skincare products.
Key Words
Lactobacillus, exosome-like nanovesicles, ELNs, senolytics, senomorphics, apoptosis
|
Study on the Mechanisms of Adipocyte Differentiation Regulation and Browning by Albizia julibrissin Bark 자귀나무 껍질의 지방세포 분화 조절 및 갈색화 기전에 관한 연구
김유나 Yuna Kim , 김태경 Tae-kyung Kim , 이문영 Moonyoung Lee
50(4) 349-358, 2024
김유나 Yuna Kim , 김태경 Tae-kyung Kim , 이문영 Moonyoung Lee
DOI:
Abstract
The subcutaneous fat layer plays a crucial role in regulating body temperature and storing energy, but its excessive accumulation can lead to obesity. This study investigated the anti-obesity potential of Albizia julibrissin bark extract (AJBE) using 3T3-L1 cells. AJBE was found to inhibit adipogenesis by reducing the expression of major adipogenic regulators, PPARγ and C/EBPα. Additionally, AJBE promoted lipolysis by enhancing the expression of proteins associated with fat breakdown, facilitating the morphological conversion of large unilocular lipid droplets into smaller multilocular ones. Furthermore, AJBE increased the expression of thermogenic and energy expenditure-related proteins such as UCP-1, inducing browning in adipocytes. This induction of browning promotes energy expenditure, potentially improving the structural characteristics of cellulite and enhancing skin appearance. This study has the potential as a new slimming and cellulite improvement material using AJBE.
Key Words
Albizia julibrissin bark extracts, cellulite, adipogenesis, lipolysis, browning
|
A Study on the In Vitro Antioxidant and Skin Cleansing Effects of Illite from Yeongdong-gun, Chungcheongbuk-do 충북 영동 일라이트의 In Vitro 항산화 및 피부 세정 효과 연구
맹지혜 Jihye Maeng , 남개원 Gaewon Nam
50(4) 359-365, 2024
맹지혜 Jihye Maeng , 남개원 Gaewon Nam
DOI:
Abstract
As part of increasing the utilization of illite produced in Yeongdong-gun, Chungcheongbuk-do as a cosmetic material, this study explored the possibility of utilizing illite raw materials by increasing the mineral content of illite powder and illite acetic acid fermentation extract, and evaluated the c leaning efficacy and safety of p roducts containing the ingredients. In vitro efficacy tests revealed that the antioxidant activity of the illite acetate fermentation extract was slightly higher than that of illite powder. In human application tests, the cleansing cream containing illite powder showed a statistically significant higher cleansing effect on fine dust and sunscreen compared to products containing traditional French clay (p < 0.05). The foam cleanser containing illite acetate fermentation extract demonstrated a cleansing efficiency of over 90%, effectively removing fine dust and sunscreen. These findings suggest that the development of fermentation and extraction processes that can increase the functionality of illite has important potential in the cosmetics industry. Future research should focus on optimizing the functional components of illite and developing new processing technologies to further expand its use as a cosmetic ingredient.
Key Words
illite, cosmetics, fermentation, new material, Yeongdong-gun
|
In Vitro Quantitative Evaluation Method for the Yoplait Phenomenon in Lip Makeups Formulation 립메이크업 제형에서의 요플레 현상에 대한 In Vitro 정량 평가법 연구
이현정 Hyun Jeong Lee , 박수지 Su Ji Park , 박소현 So Hyun Park , 장미정 Mi Jung Jang , 우영화 Young Haw Woo , 이준배 Jun Bae Lee , 박새롬 Sae Rom Park
50(4) 367-375, 2024
이현정 Hyun Jeong Lee , 박수지 Su Ji Park , 박소현 So Hyun Park , 장미정 Mi Jung Jang , 우영화 Young Haw Woo , 이준배 Jun Bae Lee , 박새롬 Sae Rom Park
DOI:
Abstract
This study is about the development of a in vitro application test method to quantify the Yoplait phenomenon that appears after using lip makeup products. The term “Yoplait phenomenon” is derived from a phenomenon in which white residue occurs on the lips immediately after using lip makeup. It is also expressed as “lip tint buildup” or “white ring” overseas, but all of these are not a official term. Since this can be unpleasant for consumers, developing test methods to quantify the phenomenon is necessary. However, the degree of Yoplait on the lips of test subjects after using the product varies between test subjects due to various external factors, and it cannot be quantified by image analysis because it occurs in the area where the lips meet. This study found that some formulations reacted with distilled water or artificial saliva and changed color, assuming that the cause of Yoplait was re-emulsification. The changes in formulation color were then analyzed through Hue values and Yoplait phenomenon (%) was derived, and trends were compared to real-world experience through a survey. This study is the first to devise a non-human application test method to quantify the Yoplait phenomenon and is expected to contribute to formulation improvement and overcoming consumer claims.
Key Words
Yoplait phenomenon, lip cosmetics, in vitro test methods, re-emulsification
|
Study on the Antioxidant and Whitening Efficacy of Extracts and Fractions Derived from Panax ginseng Seed Meal 인삼 종자박 유래 추출물 및 분획물의 항산화 및 미백 효능 평가 연구
이슬 Seul Lee , 박준성 Junseong Park
50(4) 377-385, 2024
이슬 Seul Lee , 박준성 Junseong Park
DOI:
Abstract
This study evaluated the antioxidant and whitening efficacy of 70% ethanol extract (GSEE) and solvent fractions (GSBF, GSAF) derived from ginseng seed meal to explore their potential as functional cosmetic ingredients. As a result of HPLC analysis, protopanaxadiol (PPD) was identified as a major active ingredient in GSBF, and GSBF showed DPPH and ABTS radical scavenging ability (SC50: 6.23 μg/mL, 10.36 μg/mL) along with high polyphenol content (62.57 mg/g), showing excellent antioxidant activity. Furthermore, GSBF was more than equivalent to kojic acid in tyrosinase inhibition (IC50: 20.36 μg/mL) and inhibition of melanogenesis in α-MSH-induced B16F10 cells. These findings suggest that ginseng seed meal extracts have significant potential as high-value-added cosmetic materials with antioxidant and whitening properties.
Key Words
Panax ginseng seed meal, protopanaxadiol, tyrosinase, melanin
|
Exploration and Derivation of Novel Whitening Peptides with Skin Whitening Improvement Effects 피부 미백 개선 효과가 있는 신규 미백 펩타이드 탐색 및 도출
강상문 Sang Moon Kang , 전용승 Yong-seung Joun , 이기영 Kee-young Lee , 박청 Chung Park
50(4) 387-398, 2024
강상문 Sang Moon Kang , 전용승 Yong-seung Joun , 이기영 Kee-young Lee , 박청 Chung Park
DOI:
Abstract
A peptide synthesis method and a specification and test method for five types of peptides derived from various origins were established. Various experiments were conducted to verify the skin whitening effect of peptides such as confirming skin cell toxicity, tyrosinase inhibition, and melanin production inhibition. Through the whitening improvement clinical test, the skin whitening improvement effect of the pentapeptide of the amino acid sequence of KGYSS, which was selected, was confirmed. From the above results, the new sequence of pentapeptides is considered to have the effect of improving skin whitening, and has shown potential to be used as a raw material for whitening functional cosmetics in the future.
Key Words
clinical trials, cosmetic ingredients, melanogenesis, peptide, skin whitening
|
