부반빈 Van Vinh Vu , 이경은 Kyung Eun Lee , 강상구 Sang Gu Kang

DOI:

Abstract

In this experiment, the Grateloupia elliptica (G. elliptica) was fermented using the fungus Aureobasidium pullulans (A. pullulans) and its extract was obtained from hot water and 70% ethanol solution. The extract was studied for their biological activities such as antioxidant effect, Collagenase and tyrosinase inhibition in comparison to the nonfermented exatract in same solvents. Antioxidative activity test using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) showed that ethanol extract had higher antioxidant activity than water extract. Among all of the samples, the antioxidant activity of G. elliptica fermented ethanol extracts (GEFEE) was highest. Tyrosinase inhibitory activity of GEFEE was highest with 9.8% at the 1,000 μg/mL concentration. No inhibition of collagenase from G. elliptica water extract (GEWE) and G. elliptica fermented water extract (GEFWE) was observed, but G. elliptica ethanol extracts (GEEE) and GEFEE showed increased collagenase inhibition activity with increasing concentrations of them. Collagenase inhibitory activity of GEFEE was highest with 50.3% at the 1,000 μg/mL concentration. MTS cell proliferation assay was conducted with the GEWE, GEEE, GEFWE, GEFEE and cell viability was over 90% at the 10 μg/mL ~ 1000 μg/mL concentrations for all of the samples, which suggested that the extracts were noncytotoxic. In conclusion, fermented extracts of G. elliptica could be developed to bioactive functional material for cosmetics with antioxidant and wrinkle improvement effects.

Key Words

Grateloupia elliptica (G. elliptica), fermentation, anti-oxidant, whitening effect, anti-wrinkle

이응지 Eung Ji Lee , 김잔디 Jandi Kim , 정민경 Min Kyeong Jeong , 이영민 Young Min Lee , 정용지 Yong Ji Chung , 김은미 Eun Mi Kim

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Abstract

In this study, we investigated anti-pigmentation effect of a hexapeptide. The peptide significantly reduced melanin contents and inhibited tyrosinase activity in a dose-dependent manner, in which tyrosinase is a key enzyme in melanogenesis. The peptide also significantly reduced the expression levels of tyrosinase (TYR), tyrosinase-related protein 1 (TYRP1) and their upstream transcription factor, microphthalmia-associated transcription factor (MITF). Furthermore, the peptide suppressed the phosphorylation level of cAMP-response element binding protein (CREB), a transcription factor of MITF, and increased the phosphorylation level of extracellular signal-regulated kinase (ERK), a kinase mediates MITF phosphorylation and proteasomal degradation. The peptide significantly inhibited the expression of Rab27A, Melanophilin, and MyosinVa, the components of motor complex involved in intracellular movement of melanosome. These results suggest that Hexapeptide could be used as an effective whitening agent that has inhibitory effect on melanin production and melanosome transport by regulating expression and degradation of MITF in melanocytes.

Key Words

hexapeptide, MITF, melanogenesis, melanosome transport, pigmentation

김한별 Hanbyul Kim , 명길선 Kilsun Myoung , 이현기 Lee Hyun Gee , 최은정 Eun-jeong Choi , 박태훈 Taehun Park , 안수선 Susun An

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Abstract

The skin is colonized by a large number of microorganisms with a stable composition of species. However, disease states of skin such as acne vulgaris, psoriasis, and atopic dermatitis have specific microbiome compositions that are different from those of healthy skin. The target modulation of the skin microbiome can be a potential treatment for these skin diseases. Quorum sensing (QS), a bacterial cell-cell communication system, can control the survival of bacteria and increase cell density. Also, QS affects the pathogenicity of bacteria such as biofilm formation and protease production. In this study, we confirmed anti-QS activity of Amorepacific patented ingredients, which are Lactobacillus ferment lysate (using Lactobacillus plantarum APsulloc 331261, KCCM 11179P) through bio-reporter bacterial strain Chromobacterium violaceum. The purple pigment production of C. violaceum controlled by QS was reduced 27.3% by adding 10 μg/mL of Lactobacillus ferment lysate (freeze dried). In addition, the Lactobacillus ferment lysate increased growth of Staphylococcus epidermidis 12% and decreased growth of Pseudomonas aeruginosa 38.5% and its biofilm formation 17.7% at a concentration of 10 μg/mL compared to the untreated control group. Moreover, S. epidermidis was co-cultured with the representative dermatological bacterium Staphylococcus aureus in the same genus, the growth of S. epidermidis was increased 134 % and the growth of S. aureus was decreased 13%. These results suggest that fermented lysate using Lactobacillus plantarum APsulloc 331261 may be useful as a cosmetic ingredient that can control the balance of skin microbiome.

Key Words

Lactobacillus plantarum APsulloc 331261, Lactobacillus ferment lysate, extra cellular vesicle, microbiome, Staphylococcus epidermidis, Pseudomonas aeruginosa

김미선 Mi Seon Kim , 윤경섭 Kyung-sup Yoon

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Abstract

Horse fat is known to be an effective ingredient in Asia, and the horse fat itself, which is mixed with other ingredients at the additive level, is often sold as a finished product. In this case, physical properties of the horse fat raw material are important. Many horse fats produced in Korea (Jeju) have low temperature stability, so if not stored at low temperatures, segregation may occur. In the case of Japanese horse fat, it is partially hydrogenated or is used the solid phase as the horse fat by separating the liquid phase and the solid phase that is harder and more stable than the horse fat of Jeju. In this study, the physical properties were tested to improve the temperature stability even without the partial hydrogenation process of Jeju horse fat. Various oil gelling agents were used in the study. Results confirmed that the physical properties of the hydroxystearic acid added Jeju horse fat were improved. In addition, stability evaluations at temperatures of 25 ℃, 40 ℃, 45 ℃ and flow behavior evaluations at temperatures of 25 ℃, 30 ℃, 40 ℃ were performed for Jeju horse fat with hydroxystearic acid, 100% Jeju horse fat, and 100% Japanese horse fat. Results showed that the Jeju horse fat improved in flow behavior by adding hydroxystearic acid similar to that of Japanese horse fat. In addition, when the crystal state was observed under a microscope, the thermal stability was improved by decreasing the size of the needle-type crystals with the addition of hydroxystearic acid. Jeju horse fat containing hydroxystearic acid was found to have no physical problems even when stored at room temperature for a long time.

Key Words

horse fat, oil gelling agent, viscosity, hydroxystearic acid, stability, Jeju, Japan

우병영 Byoung Young Woo , 신송석 Song Seok Shin , 홍용덕 Yong Deog Hong , 주영협 Yung Hyup Joo , 정연수 Yeonsu Jeong , 이범진 Beom-jin Lee , 김수동 Soo-dong Kim

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Abstract

A novel series of adamantyl derivatives of N-aryl amides as androgen receptor antagonists were synthesized and their anti-androgenic activities were evaluated. The N-aryl amides containing adamantyl derivatives had more activity than those lacking adamantyl substitutions. The synergistic effect of bulkiness of the adamantyl group and modulation of electron density on the aromatic ring by pendant groups was crucial to the potent antagonism. Molecular modeling studies were performed to elucidate the interactions between ligands and receptors.

Key Words

androgen receptor, admantyl N-aryl amide, hair loss

박승희 Seung Hee Park , 이정민 Jeong Min Lee , 이승호 Seung Ho Lee

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Abstract

This study was conducted to establish optimal high pressure liquid chromatography (HPLC) conditions for estimation of the ergothioneine contents in the three kinds of water extracts of Pleurotus eryngii var. ferulae (Meaksong, Beesan No. 2, Baekwhang). By comparing the four different HPLC conditions, optimun condition for quantifying the contents of ergotioneine was established (shodex HILIC column, 35 ℃, 1.0 mL/min). By this method, the contents of ergothioneine in Meakong (3.04 ± 0.02 mg/g), Beesan No. 2 (3.15 ± 0.05 mg/g) and Baewhang (1.13 ± 0.07 mg/g) were estimated. DPPH and ABTS radical scavenger activities of these three kinds of Pleurotus eryngii var. ferulae were estimated and the contents of total phenol and flavonoid were also estimated. Taken together, this study establish an optimun HPLC condition for determining the ergothioneine contents in water extracts of Pleurotus eryngii var. ferulae. Furthermore water extracts of Maesong and Beesan No. 2 showed relatively high contents of ergothioneine and antioxidant activity, suggesting that these materials could be used as potential antioxidant in developing functional cosmetics.

Key Words

antioxidant activity, Ergothioneine, HPLC analysis, Pleurotus eryngii var. ferulae

송상훈 Sang-hun Song , 임병택 Byung Tack Lim , 손성길 Seong Kil Son , 강내규 Nae-gyu Kang

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Abstract

To prevent loss of hair protein during hair washing process by water through, a shield coating the pathway of water molecules was studied. Hydrophobic virgin hair, hydrophilic hair, which was damaged only methyleicosanoic acid (18-MEA) on the surface, and a repaired hair re-bound 18-MEA, were prepared and water mass changes by as heat were measured. Results showed that hydrophobic hairs followed bi-exponential function of 39 s and 151 s and other two hairs exhibited fast- and mono-exponential decay with 83 s, reflecting the extraction of water molecules without any resistance at the hydrophobic surface. On the assumption that hydrophobic surface resists an extraction of protein in water during the wash, the protein concentrations were compared from the hair of hydrophobic and hydrophilic surface. The extracted hair proteins were 179 and 148 μg/mL from the hair coated with hydrophilic polyethylene glycol (PEG) and hydrophobic polydimethylsiloxane (PDMS), respectively. This study suggested that hydrophobic coating on the hair surface could be used to prevent protein loss in wash, represented by LFM. In conclusion, this research provides some useful information to contribute to the development of hair washing products that can prevent protein loss in the cleaning process by granting hydrophobic coatings.

Key Words

oil delivery, hair, shampoo, protein protection, moisture

정연수 Yeon Su Jeong , 이진영 Jin Young Lee , 고재영 Jaeyoung Ko

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Abstract

In this study, we attempted to obtain an active ingredient that inhibits the production of matrix metalloproteinase-1 (MMP-1) that breaks down collagen in human skin fibroblasts. More than 50 plant extracts were screened, and Lonicera japonica was selected for this study. The stem of L. japonica was extracted with 70% ethanol and fractions with solvents in the order of hexane, ethyl acetate, and butanol. MMP-1 production were significantly inhibited at the concentration of 50 μg/mL of the ethyl acetate layer and 200 μg/mL of the butanol layer. To get a fraction containing all of these effective components, 80% ethanol fraction (LJ F80) was obtained through HP20 resin column chromatography. The reference substance, loganin and LJ F80 inhibited dose-dependently MMP-1 production. At the same concentration, LJ F80 showed a higher inhibitory effect than loganin. The stability of this fraction was analyzed with HPLC while kept storing at 4 ℃, room temperature, and 40 ℃, for 16 week. The stability was maintained as ± 10% of initial value with reference loganin. Therefore, it is thought that LJ F80 of L. japonica may be used to improve wrinkles.

Key Words

Lonicera japonica, Matrix metalloproteinase-1, Loganin, Stability, Anti-wrinkle

이성훈 Sung Hoon Lee , 손의동 Eui Dong Son , 최은정 Eun-jeong Choi , 박원석 Won-seok Park , 김형준 Hyoung-june Kim

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Abstract

Psychological stress can affect the physiological condition of the skin and cause various cutaneous disorders. The stress hormone cortisol is secreted by various skin cells such as fibroblasts, keratinocytes, and melanocytes. Tight junctions (TJs) are cell-cell junctions that form a barrier in the stratum granulosum of mammalian skin. TJs can also affect other skin barriers and are affected by chemical, microbial, or immunological barriers. Stress can cause damage to the skin barrier. Interestingly, to our knowledge, there has not been any research demonstrating the involvement of TJs in this process. In this study, cortisol was used to treat keratinocytes to determine its role in regulating TJs. We found that cortisol damaged skin barrier function by regulating the gene expression and structure of TJ components. Cortisol also inhibited the development of the granular layer in a skin equivalent model. These results suggest that cortisol affects the skin barrier function by the regulation of TJs.

Key Words

cortisol, tight junction, skin barrier

신재영 Jae Young Shin , 김윤선 Yun Sun Kim , 안영제 Young Je Ahn , 강내규 Nae-gyu Kang , 이상화 Sang Hwa Lee

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Abstract

Diesel particulate matter (DPM) induces inflammatory cytokines in HaCaT and stimulates XRE promoter activity through AhR binding. Thus, it increases CYP gene family expression. In this study, we have elucidated inhibitory effect of LG Herbal Medicine Complex (LG-HMC) on DPM-induced XRE promoter activity and inflammatory cytokine expression. First of all, the XRE promoter activity was overexpressed by DPM treatment and the LG-HMC abrogated the XRE promoter activity. Morus alba bark extract, Scutellaria baicalensis root extract and constituents of LG-HMC, were found to be main effecters against DPM induced XRE promoter activation. We also found that DPM treatment elevated inflammatory cytokines in HaCaT and the treatment of LG-HMC, Morus alba bark extract and Scutellaria baicalensis root extract down-regulated the DPM induced inflammatory cytokine expression. Additionally, Morus alba bark extract and Scutellaria baicalensis root extract were found to act as free radical scavengers. In conclusion, we confirmed skin protective effect of LG-HMC and uncovered two components, Morus alba bark extract and Scutellaria baicalensis root extract, that play major role in protecting epidermal kertinocytes from damage.

Key Words

diesel particulate matter, HacaT, inflammation, Xenobiotic response element, herbal medicine complex