Vol.27 No.2
화장품과 생명공학김재민 27(2) 5-5, 2001
Title화장품과 생명공학
김재민 DOI: AbstractKey Words |
Biomedical research에서의 cDNA Microarray의 적용이용성 27(2) 7-13, 2001
TitleBiomedical research에서의 cDNA Microarray의 적용
이용성 DOI: AbstractKey Words |
High Throughput Biotechnologies and Their Applications for the Cosmetic Research
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한국인의 피부노화 특징 및 발생기전정진호 27(2) 31-35, 2001
Title한국인의 피부노화 특징 및 발생기전
정진호 DOI: AbstractKey Words |
해양으로부터의 기능성 화장품 소재 개발 동향이홍금 27(2) 37-43, 2001
Title해양으로부터의 기능성 화장품 소재 개발 동향
이홍금 DOI: AbstractKey Words |
산삼의 배양 및 그 응용에 관한 연구신미희 27(2) 45-56, 2001
Title산삼의 배양 및 그 응용에 관한 연구
신미희 DOI: AbstractKorea mountain ginseng known as oriental miracle drug is an important medicinal plant. The effect of mountain ginseng adventitious roots extract has been described. The valuable root of mountain ginseng contained several kinds of ginsenosides that have been confirmed to have many active functions for the human body. However, the study of mountain ginseng has a limit because the price of wild ginseng is very expensive and rare. The mountain ginseng adventitious roots were derived from mountain ginseng callus that were induced from mountain ginseng roots. Adventitious roots were separated from callus and grown in solid media(Murachige and stoog media). It was cultured in a 20L bioreactor. After culturing for 40days, adventitious roots were harvested. Afterwards the harvested mountain ginseng adventitious roots were dryed and extracted. We examined the effect on melanogenesis of mountain ginseng adventitious roots extract. Here, we report the inhibitory effect of melanin biosynthesis on the adventitious roots extract of In vitro test. Also, we assessed the safety of adventitious roots extract. In vitro, cytotoxicity of adventitious roots extract was assessed in mouse fibroblast using two method: The neutral red uptake assay and the MTT assay. In vivo, the allergic and irritant were patch tested in 30 patients. Consequently, extract of mountain ginseng adventitious roots have inhibitory effect on melanin biosynthesis in B-16 melanoma cell test, tyrosinase inhibitory test and DOPA auto-oxidation test. There were decreased 86%(0.5% concentration)，45%(1% concentration) and 61%(1% concentration), respectively Key Words |
Antimicrobial Activity of BiosurfactantsDalsoo Yoo , Kapjung Kim , Youngbum Kim , Eunki Kim 27(2) 57-58, 2001
TitleAntimicrobial Activity of Biosurfactants
Dalsoo Yoo , Kapjung Kim , Youngbum Kim , Eunki Kim DOI: AbstractBiosurfactant has antibiological activities. Due to its low toxicity, biosurfactant can be applied to human health care products. Posseing the antibiological activities as well as the surfactant properties implies that biosurfactant can be widely used in the cosmetic industry. In tiiis presentation, applications of biosurfactant have been reviewed including the antimicrobial activities of sophorolipid. Sophorolipid，a biosurfactant produced from Candida bombicola ATCC 22214，showed antimicrobial activity against Bacillus subtilis，Staphylococcus xylosus，Streptococcus mutans, and Propionibacterium acne at 4, 1，1，0.5 ppm as MIC (minimum inhibitory concentration^ Also 100 ppm of sophorolipid inhibited 50% of cell growth of plant pathogenic fungus, B. cinera. However, sophorolipid showed no effect on the Escherichia coli.，indicating its selective antimicrobial activity depending on the cell wall structure. Treatment of B. subtilis with sophorolipid increased the leakage of intracellular enzyme, malate dehydrogenase, indicating the possible interaction of sophorolipid with cellular membrane. Between lactone-type and acid-type sophorolipid, the former showed higher antimicrobial activity. Key Words |
Safety & Efficacy Testing of Cosmetic Ingredients using Cultured Human Skin / Cornea Tissue Model
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