이명희 , 최종근 , 이보섭 , 김상진

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이명희

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Abstract

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박병덕 Byeang Deog Park , 흥선영 Seon Young Hong , 정세규 Sekyoojeong , 조인식 In Sik Jo , 구형서 Hyeong Seo Gu , 한일민 Ii Min Han , 이상명 Sang Myeong Lee , 이완규 Wan Kyu Lee

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We have tried to measure the anti-dandruff effect of the several kinds of formulations by determining the MIC values of the P. ovale which was determined by resazurin(alarmar Blue™). To get high reproducibility, it was suggested that about 2.6 X 105cfu/mℓ of P. ovale should be incubated with alarmar Blue™, optimum dilution ratio between alarmar Blue™ and PBS buffer should be 1:1 - 1:4, and oplimam incubation time should be 16 ~24 hours. Even though 1:1 diluted alarmar Blue™ was incubated with P. ovale，the metabolic activity of P. ovale was not inhibited by alarmar Blue™. The Minimum Inhibitory Concentration(MIC) values of several kinds of anti-dandruff formulation which were the mixture system between Zinc pyrithione and Climbazole make it possible to determine the optimal anti-dandruff formulation, which show similar results with that of microscopic MIC determination and that of SDDM(Skin-Disk Diffusion Method). It is expected that the anti-dandruff test method which uses alarmar Blue™ could be used as an effective in vitro test method because it was not so much affected by the turbidity of the broth and samples, and it can afford the MIC values of many samples within relatively short time by using microplate reader.

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이강연 Kang Yun Lee , 한창규 Chang Giu Han , 조춘구 Choon Koo Zhoh

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Ascorbic acid which has various physiological benefits as the functional substance is easily oxidized and destroyed by the structural instability. Liposome encapsulated pure ascorbic acid was prepared for the sake of the constraint of oxidation. The influence of cholestrol or β-sitosterol on the stabilization of liposome was investigated. Butylated hydroxytoluene(BHT), tertiary butylhydroquinone(TBHQ), α-glycosyl rutin and natural concentrated tocopherol were used for constraint of oxidation of ascorbic acid. The presence of cholesterol or -sitosterol decreased oxidation of ascorbic acid. That results were thought that cholesterol or β-sitosterol so increased rigidity of bilayer that the leakage of vitamin C decreased. As a result, the oxidation and degradation of vitamin C were constrained. At 0.3w/w% cholesterol content the most stable liposome was formulated. The whole antioxidant that used at the research constrained oxidation of ascorbic acid. The antioxidation for ascorbic acid increased in order of tertiary butylhydroquinone, α-glycosyl rutin, butylated hydroxytoluene and natural concentrated tocopherol. But α-glycosyl rutin is preferable to tertiary butylhydroquinone which was the most effective in antioxidation as the antioxidant of ascorbic acid which was utilized in cosmetics and pharmacy.

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조춘구 Cboon Koo Zhoh, , 한창규 Chang Giu Han

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Manuka oil named New Zealand’s tea tree oil is oil-soluble and comes from nature. Manuka oil and its extract α-pinene, Oxy’less clear, R-limonene which is one of the component of Citrex extracted from Grapefruit seed and Citrex were used to estimate the antimicrobial activity and to improve the capability of antiseptic. Disk diffusion method was used to measure the antimicrobial activity. Escherichia coli which is gram-negative bacteria and Staphylococcus aureus which is gram-positive bacteria were used as strain. The antimicrobial activity of Manuka oil and α-pinene for Escherichia coli, Staphylococcus aureus was similar when the concentration of Manuka oil and α-pinene are 10㎕/paper disk. However, antimicrobial activity of Manuka oil for Escherichia coli, Staphylococcus aureus was better than that of a -pinene when the concentration of Manuka oil and α-pinene was low. Antimicrobial activity of Oxyless clear is better than that of propyl para hydroxybenzoate(PPHB), R-limonene at all the concentration and is similar to that of α-pinene. Antimicrobial activity of Oxy'less clear is lower than that of Manuka oil and Citrex. Antimicrobial activity of Citrex is superior to that of R-limonene. As a result, the antimicrobial activity of Manuka oil and Citrex for two strains is better than that of the other antiseptics. The proper ratio of Maunka oil and Citrex can improve the antimicrobial activity. The proper ratio obtained from this study was 75% of Maunka oil and 25% of Citrex for Escherichia coli, 25% of Maunka oil and 75% of Citrex for Staphylococcus aureus.

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이현주

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The history of make-up can go back to the beginning of human being and it ean’t be separated from women's life. The change of history and women’s position have given make-up different form and meanings. So make-up becomes another text which can read social · cultural specialty. This study has tried to find out make-up as women culture for women have experienced make-up in a specific situation for a long time and a suit of make-up experience from self-retrospect and made an open channel for women and this paper checked out the possibility of alternative make-up culture. This research used new audience theory of cultural studies which is used for communication study and studied active meaning-construction process and its resistance pleasure. This study saw women as independent subjects not passive victims and how make-up has been different meanings in women's life. And what pleasure is made and how the way of resistance made constructed in the regulation of make-up.

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