Soo-nam Park , Sang- Won Choi , Yong-chool Boo

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Abstract

The major flavonoid component of Ginseng leaf is trifolin, a glycoside of kaempferol. To evaluate the antioxidative properties of trifolin and kaempferol on cellular membranes, we compared them with the other flavonoids through the ¹O₂-induced photohemolysis of rabbit erythrocytes. All the flavonoid aglycones including kaempferol, quercetin and baicalein protected effectively the cells from the ¹O₂-caused damage in a dose-dependent manner, by scavenging ¹O₂ and free radicals in the cellular membranes. The solubilization of the flavonoid aglycones into micelles or erythrocyte membranes was deduced from spectrophotometric and microscopic observations. The flavonoid glycosides were not protective or less protective than their corresponding aglycones, and trifolin was the only glycoside that exhibited a solubilization into the membranes and a significant protection against the photohemolysis. We also tested some phenolic compounds contained in Ginseng, and found that they did not prevent the photohemolysis so effectively as kaempferol or trifolin.

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백병기 Baik Beong Ki , 한창규 Han Chang Giu , 이영운 Lee Young Woon

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Abstract

In this report, the variation of skin surface between before treatment and after treatment was investigated by skin image analyzer. The 30 volunteers were applied cream and lotion on the forearm inside for 4weeks, and the measurement parameter of skin surface was adopted peak, length, height, and width. The results were as follows ; 1. Variation trends of parameters was showed that number of peaks were increased, but length, height and width were decreased. 2. The order of improvement for skin surface are as follow ; height > length > peak > width. 3. And also disclosed that the number of peak has a correlation with width, and length has a correlation with height.

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이보섭 Lee Bo-seaub , 김진우 Kim Jin-woo

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Abstract

Angelica gigas extract and Cnidium officinale extract in Bath essence were determined by high performance liquid chromatography with a UV detector by use of Bondapak C-1B column. Decursin and Ligustilide were used as indicator ingredient for analysis of Angelica gig as extract and Cnidium officinale extract. Each indicator ingredient was isolated from each plant and purified using silver-nitrate treated silica column. Their structure were conformed with IR, NMR and HPLC. The recoveries of Angelica gigas extract and Cnidium officinale extract added to Bath essence sample were 96. 5-109.5% and 112-115%

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임호빈 Lim Ho -bin , 최상원 Choi Sang -won , 김진우 Kim Jin -woo

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Abstract

A method was described for the analysis of Artemisia extract in cosmetic soap. Esculetin-6-methylether was used as indicator ingredient for analysis of Artemisia extract. It was isolated from the plant and purified with silicagel column. The structure was conformed with IR, NMR and MASS spectroscopy. The Artemisia extract in sample was isolated with chloroform and interfering com- pounds were eliminated with silicagel column. The Artemisia extract was determined by reverse phase high-performance liquid chromatography with a fluorescence detector and a solvent system of H₂O/THF/MeOH. The recorveries of Artemisia extract were 93.9-106.1% in the cosmetic soap.

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Kim Jong-il , Lee Joa-hoon , Lee Yoo-young , Kim Chang-kew

DOI:

Abstract

Image analyser was used to understand the condition of skin surface and to evaluate the efficacy of cosmetic treatment. It was unsatisfactory to analyse skin surface structure although several methods using image analyser had been presented. We developed the new system composed of image analyser and Tandem Scanning Reflected Light Microscope (TSRLM) having the remarkable optical sectioning property as image input device. By using this new system, we quantitatively measured the change of skin surface, the depth and width of furrow in micron unit, resulted by cosmetic treatments. And also three dimensional image of skin was reconstructed with serial sectioned images, which were captured through TSRLM, for better understanding of the effect of cosmetic treatment. It was found that skin relief was more easily understood and the change of skin surface caused by cosmetic treatment was more accurately measured by using this system. In addition, we was also aware of the possibility of in vivo direct measurement of skin furrow without replica. It was conceivable that our system could be applicable for study of cosmetic effects further.

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