Hai-joo Yang , Neung-an Lee

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Abstract

We have con finned the photo toxicity of orange flower absolute, studied the phototoxic ingredient and developed a method to eliminate it. To confirm the phototoxicity, we tested French orange flower absolute, Moroccan absolute and Egyptian absolute with Hartley strain guinea pig under UV irradiation ranging from 320-400 nm supplied with fluorescent lamps. Using a combination of isolation techniques including HPLC and IR, we were able to confirm that the phototoxic agent was bergapten. The development of a non-phototoxic orange flower absolute using ion exchange chromatography is described.

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박수남 , 이현태 , 한기태

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Abstract

Production of shikonin derivatives through cell suspension culture of Lithospermum erythrorhizon was investigated. Optimal concentrations of IAA and kinetin on the growth of cell suspension were 0.2 and 0.1 ppm respectively. Pigment content was markedly increased when a1uminum oxide was added to the production medium and its optimal concentration was 1.5g/70ml medium. The most effective concentration of IAA was 0.5 ppm and the production of pigment did not depend on the kinetin concentration.

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정원범 , 고종성

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Abstract

Silica spheres were prepared by interfacial reaction method. Factors influencing to the mean particle size and specific surface area of silica spheres were investigated. The experiment about the surface modification of silica spheres was carried out. It was observed that silica spheres have characteristics of the spherical shape with the vacancy in. the inner side, high surface area, and reaction tendency by many silanol group. The mean particle size of silica spheres is dependent on the surfactant concentration and W/O ratio. The specific surface area is influenced by SiO₂/Na₂O mole ratio in sodium silicate. Silica spheres coated with titanium dioxide or zirconium dioxide improve the DV protection effect. Titanate and silane coupling agent make chemical bond with silica surface and improve the organophile and the dispersibility of silica spheres.

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양창모

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Abstract

Purely synthesized L-ascorbic acid 2 phosphate Mg salt (1 AsA PMg) improved the weak point of ascorbic acid which is easily decomposed in water solution. This compound is hydrolyzed with phosphatase of skin to corresponding ascorbic acid giving Vitamine C activities. The buffer solution of potassium acetate 0.5% and citric acid 0.005% and the sodium sulfite respectively showed good stabilizing effect of the AsA PMg solution. Compared to the other ascorbic acid derivatives the good solubility of AsA PMg gives broad application to cosmetic field.

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양승각 , 전기붕

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Abstract

Seven bacterial strains capable of conversing Gardenia irridoidglucoside into blue color was isolated on nutrient agar plates with 0.1 % water extracted solution of Gardenia's dryed seed. In the seven, strain No. C₂ was most effective in the production of blue color. The optimal conditions in production of blue color were when initial pH of medium was 7.0 and cultivation temperature was 35℃. In 5ℓ-Jar fermantor, the powder of blue color was produced about 15% (W /W). And the color was relatively stable in our test.

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朴秀男 , 李大衡 , 李泰寧

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Abstract

β-Carotene has been known as an effective quenching agent of singlet oxygen and the carotenoid pigments in general are expected to protect cells against photosensitized oxidations. We are determined the quenching rate constants of several Ketocarotenoids including capsanthin, capsanthin diester. astaxanthin and fucoxanthin, and the relative quenching activities against singlet oxygen were compared with those of β-carotene and reported carotenoids. Nevertheless the ketocarotenoids exhibited lower quenching rate constants than β-carotene, they showed more pronounced protective activitives than β-carotene against photohemlysis induced by singlet oxygen. Among the ketocarotenoids investigated, fucoxanthin indicated a significant protective activity for the cell. The results suggested that. 1) ¹O₂ may be alikely initiator of photohemolysis, but this reaction is followed by slow dark reactions involving secondary reactive species. 2) For protection of RBC against photodynamic action with carotenoids, carotenoids having functional groups such as -C=O and -OH groups are most efficient. This suggests that partition of carotenoids between the bulk and the membrane and/or their specific binding to membrane proteins are more critical for the photo-protection by carotenoids than is a diffusional quenching of ¹O₂.

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