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ISSN : 1226-2587(Print)
ISSN : 2288-9507(Online)
Journal of the Society of Cosmetic Scientists of Korea Vol.45 No.1 pp.87-93
DOI : http://dx.doi.org/10.15230/SCSK.2019.45.1.87

Analytical Method Development of (−)‑Epicatechin gallate in Penthorum chinense Pursh Extract using HPLC

Jin Gwan Kwon*,Yeon Woo Jung*,Changon Seo*,Seong Su Hong*,Chun Whan Choi*,Ji Eun Lee*,Hyun Tak Shin**,Su Young Jung**,Jin Kyu Kim*†
*Gyeonggido Business and Science Accelerator, Gyeonggi-do, Suwon, Yeongtong-gu, Gwanggyo-ro 147, 16229, Korea
**DMZ Botanic Garden, Korea National Arboretum, Gyeonggi-do, Pocheon-si, Gwangneungsumogwon-ro 415, 11186, Korea
주 저자 (e-mail: jinkyu90@gbsa.or.kr
call: 031)888-6130

Abstract

This study attempted to eatablish a High Performance Liquid Chromatography (HPLC) analysis method for the determination of (−)‑epicatechin gallate as a part of the quality control for the development of functional cosmetic materials from Penthorum chinense Pursh. HPLC was performed on a Unison US-C18 column (4.6 × 250 mm, 5 μm) with a gradient elution of 0.05% (v/v) trifluoroacetic acid (TFA) and methyl alcohol at a flow rate of 1.0 mL/min at 30 ℃. The analyte was detected at 280 nm. The HPLC method was performed in accordance with the International Conference on Harmonization (ICH) guideline (version 4, 2005) of analytical procedures with respect to specificity, precision, accuracy, and linearity. The limits of detection and quantitation were 0.11 and 0.33 mg/mL, respectively. Calibration curves showed good linearity (r2 > 0.9999), and the precision of analysis was satisfied (less than 0.6%). Recoveries of quantified compounds ranged from 99.51 to 101.92%. This result indicates that the established HPLC method is very useful for the determination of marker compound in P. chinense Pursh extracts.

HPLC를 이용한 낙지다리 추출물의 (−)Epicatechin

권진관*,정연우*,서찬곤*,홍성수*,최춘환*,이지은*,신현탁*,정수영*,김진규*†
*(재)경기도경제과학진흥원
**국립수목원

초록

본 연구는 낙지다리(Penthorum chinense Pursh) 추출물을 기능성 화장품소재로 개발하기 위해 (−)‑epicatechin gallate를 지표성분으로 선정하고, 품질관리를 위해 High Performance Liquid Chromatography (HPLC)를 이용하여 분석법을 개발하였다. 분석에 사용된 칼럼은 Unison US-C18 (4.6 × 250 mm, 5 μm, Imtakt, USA)을 사용하여 0.05% (v/v) trifluoroacetic acid (TFA)와 메탄올을 이동상 조건으로 컬럼 온도는 30 ℃ 에서 유속은 1.0 mL/min 로 검출파장은 280 nm에서 검출하였다. International Conference on Harmonization (ICH) 가이드라인(version 4, 2005)을 근거로 하여 특이성, 직선성, 정밀성, 정확성, 검출한계 및 정량한계를 분석하여 분석방법을 검증하였다. 검출한계 및 정량한계는 각각 0.11 mg/mL 및 0.33 mg/mL로 나타났으며, 검량곡선은 상관계수값이 0.9999로 양호한 직선성을 보였고, 정밀성 분석결과 도 0.6% 이하로 확인하였다. 또한, 회수율은 99.51 ~ 101.92% 범위로 정확성이 있음을 알 수 있다. 따라서, 본 분석법은 낙지다리 추출물의 지표성분의 분석법은 적합한 시험법임이 검증되었다.

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