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ISSN : 1226-2587(Print)
ISSN : 2288-9507(Online)
Journal of the Society of Cosmetic Scientists of Korea Vol.44 No.3 pp.219-229
DOI : http://dx.doi.org/10.15230/SCSK.2018.44.3.219

Studies on Cosmeceutical Activity of Extracts of Moringa oleifera Extract

So Ra Kim, Dan Hee Yoo, Hyeon Ji Yeom, Min Jeong Oh, Jin Young Lee
Department of Cosmetic and Biotechnology, Hoseo University
주 저자 (e-mail: jylee@hoseo.edu)
call: 041)540-9552

Abstract

The purpose of this study was to investigate the role of the Moringa oleifera (M. oleifera) extract as a cosmetic additive. The tyrosinase and elastase inhibitory effects showed 47% and 39% at 1,000 μg/mL concentration, respectively. Also, the collagenase inhibition effect was 31% at 500 μg/mL concentration. A cell viability test, measured on macrophage cell (RAW 264.7) and melanoma cell (B16F10) by ethanol extract of M. oleifera, showed 94.2% and 94.8% at 100 μg/mL concentration, respectively. In order to confirm anti-inflammatory activity, we examined the inhibitory effects on the production of lipopolysaccharides (LPS)-induced NO in RAW 264.7 cells by Griess assay. As a result, the M. oleifera extract showed a concentration-dependent inhibition of NO production. The protein expression inhibitory effects of M. oleifera extract were measured by western blot at 25, 50, 100 μg/mL concentration and the β-actin. Results showed that the expression inhibition rates of the iNOS, COX-2, MITF, TRP-1, TRP-2, tyrosinase protein were decreased by 85.8%, 57.5%, 80.7%, 30%, 29.9%, 23.6% at 100 μg/mL concentration, respectively. It was concluded that M. oleifera extracts had the anti-inflammatory and whitening effects and thus could be applied for cosmetics as a natural ingredient.

모링가 추출물에 대한 화장품약리활성 검증

김소라, 유단희, 염현지, 오민정, 이진영
호서대학교 화장품생명공학부

초록

본 연구에서는 화장품 천연소재로서 모링가 에탄올 추출물의 이용 가능성을 확인하였다. Tyrosinase와 elastase 저해활성을 측정한 결과 각각 1,000 μg/mL에서 47%, 39%의 활성을 나타내었다. 모링가 에탄올 추출물에 대한 collagenase 저해활성을 측정한 결과 1,000 μg/mL에서 31%의 활성을 확인하였다. 세포 생존율을 MTT 분석법으로 확인한 결과 대식 세포(Raw264.7)와 멜라노마 세포(B16F10)의 농도 구간이 100μg/mL 일 때 각각 94.2%, 94.8%의 생존율을 보였다. 항염증 활성을 확인하기 위해 griess 분석에 의하여 대식세포에 lipopolysaccharides (LPS)를 처리하였다. 그 결과 모링가 에탄올 추출물의 농도가 증가함에 따라 NO 발현 억제효과를 확인하였다. Western blot을 통한 단백질 발현 억제 효과를 측정하기 위해 25, 50, 100μg/mL 농도의 모링가 에탄올 추출물과 β-actin을 사용하였다. 그 결과, iNOS, COX-2, MITF, TRP-1, TRP-2, tyrosinase의 단백질 발현양이 100 μg/mL에서 85.8%, 57.5%, 80.7%, 30%, 29.9%, 23.6%로 억제됨을 확인하였다. 따라서 미백 및 항염증 효과가 우수함을 확인하였고, 모링가 에탄올 추출물의 화장품 소재로서의 가능성을 확인하였다.
 

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